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Image Search Results
Journal: Scientific Reports
Article Title: Nucleoside-Diphosphate-Kinase of P. gingivalis is Secreted from Epithelial Cells In the Absence of a Leader Sequence Through a Pannexin-1 Interactome
doi: 10.1038/srep37643
Figure Lengend Snippet: NDK was visualized by transmission electron microscopy using immunogold labelling and rabbit anti- P. gingivalis NDK antibody. NDK (blue arrows) is seen on the P. gingivalis bacterial surface and in the host cytoplasm independently of the bacteria ( A,B ). An enlarged image of the boxed area is shown to the right ( A ). P. gingivalis with no primary antibody incubation ( C ), ndk- deficient mutant strain, ΔNDK ( D ) and GECs without infection ( E ) with both primary and secondary antibody incubations were used as controls. The black arrows point to non-specific background level of gold labelling staining in the control samples. Bar represents 1 μm.
Article Snippet: Coverslips with fixed cells were mounted onto
Techniques: Transmission Assay, Electron Microscopy, Bacteria, Incubation, Mutagenesis, Infection, Staining, Control
Journal: Journal of Nanobiotechnology
Article Title: Targeting of ICAM-1 on vascular endothelium under static and shear stress conditions using a liposomal Gd-based MRI contrast agent
doi: 10.1186/1477-3155-10-25
Figure Lengend Snippet: Binding of aICAM-1 L to bEnd.5 in the competing presence of shear stress. TNFα-activated cells incubated with L or aICAM-1 L for 2 h at 37 °C and shear stress values of 0, 0.25 or 0.5 Pa. (a) Fluorescence microscopy of rhodamine lipids in liposomes adherent to bEnd.5 cells (red). Scale bar = 100 μm. (b) Cellular rhodamine fluorescence intensity quantified with FACS. * = p < 0.05 vs. all groups, ANOVA with Bonferroni correction, ** = p < 0.05 vs. IgG L, t -test. n = 2-5. (c) ICAM-1 expression at different shear stress levels. * = p < 0.05 vs. 0 Pa, ANOVA with Bonferroni correction. n = 3.
Article Snippet: The bEnd.5 cells were cultured and activated with TNFα on
Techniques: Binding Assay, Shear, Incubation, Fluorescence, Microscopy, Liposomes, Expressing