Journal: ACS nano

Article Title: Tunable Macroscopic Alignment of Self-Assembling Peptide Nanofibers

doi: 10.1021/acsnano.4c02030

Figure Lengend Snippet: Valvular interstitial cell spreading on aligned K2 hydrogels. (A) Day 1, (B) day 3, and (C) day 7 confocal microscopy images of VICs on (i) pre-gelled, (ii) 1× PB, (iii) 3× PB, and (iv) 5× PB hydrogels (DAPI = blue, F-actin = green; scale bars = 300 μm). All images are maximum intensity projections of Z-stacks that have been cropped and rotated so the direction of K2 fibrous alignment is horizontal. Polar histograms to the right of each image display the Fourier gradient structure tensor calculated using the F-actin channels, where the direction of K2 fibrous alignment is 0°. (D) Day 1, (E) day 3, and (F) day 7 comparisons of (i) resultant vector lengths calculated from Fourier gradient structure tensors (n = 3 images; line at mean; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett’s multiple comparison tests) and (ii) nuclear angle distributions with respect to the angle of K2 fibrous alignment (n = 3 images with between 294 and 1854 pooled nuclei; center line, box bounds, and whiskers indicate the median, first and third quartiles, and 10 and 90 percentiles, respectively; **P < 0.01, ***P < 0.001, ****P < 0.0001 by Kolmogorov–Smirnov test). (G) Day 3 and (H) day 7 confocal microscopy image stitches of VICs on 1× PB hydrogels (DAPI = blue, F-actin = green; scale bar = 500 μm).

Article Snippet: Samples were imaged on standard glass microscopy slides (Fisher Scientific, Pittsburgh, PA) at a consistent angle.

Techniques: Confocal Microscopy, Plasmid Preparation, Comparison

Journal: Journal of Nanobiotechnology

Article Title: Targeting of ICAM-1 on vascular endothelium under static and shear stress conditions using a liposomal Gd-based MRI contrast agent

doi: 10.1186/1477-3155-10-25

Figure Lengend Snippet: Binding of aICAM-1 L to bEnd.5 in the competing presence of shear stress. TNFα-activated cells incubated with L or aICAM-1 L for 2 h at 37 °C and shear stress values of 0, 0.25 or 0.5 Pa. (a) Fluorescence microscopy of rhodamine lipids in liposomes adherent to bEnd.5 cells (red). Scale bar = 100 μm. (b) Cellular rhodamine fluorescence intensity quantified with FACS. * = p < 0.05 vs. all groups, ANOVA with Bonferroni correction, ** = p < 0.05 vs. IgG L, t -test. n = 2-5. (c) ICAM-1 expression at different shear stress levels. * = p < 0.05 vs. 0 Pa, ANOVA with Bonferroni correction. n = 3.

Article Snippet: The bEnd.5 cells were cultured and activated with TNFα on flow chamber microscopy slides (50x5x0.8 mm 3 μ-slide, Ibidi GmbH) under static conditions.

Techniques: Binding Assay, Shear, Incubation, Fluorescence, Microscopy, Liposomes, Expressing